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spatial gene expression profiling xenium in situ technology  (10X Genomics)

 
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    Structured Review

    10X Genomics spatial gene expression profiling xenium in situ technology
    Spatial Gene Expression Profiling Xenium In Situ Technology, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+expression+gene+expression+profiling/pm42288489-252-0-9
    Average 86 stars, based on 1 article reviews
    spatial gene expression profiling xenium in situ technology - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Expressing:

    Article Title: Dysregulated lymphocyte localization in idiopathic multicentric Castleman disease
    Article Snippet: .. To determine the source of elevated chemokine expression in iMCD-TAFRO lymph nodes, we performed single-cell gene expression profiling of gently dissociated FFPE tissue using 10X Genomics’ FLEX pipeline. ..

    Single Cell:

    Article Title: Dysregulated lymphocyte localization in idiopathic multicentric Castleman disease
    Article Snippet: .. To determine the source of elevated chemokine expression in iMCD-TAFRO lymph nodes, we performed single-cell gene expression profiling of gently dissociated FFPE tissue using 10X Genomics’ FLEX pipeline. ..

    Article Title: ROR1-PI3K/AKT signaling drives adaptive resistance to cell cycle blockade in TP53 mutated ovarian cancer
    Article Snippet: .. Single-cell gene expression profiling was conducted using the 10x Genomics Chromium Single Cell 3’RNAseq platform (10x Genomics, # CG000317 ). ..

    Article Title: CMAS dampens anti-tumor immunity and associates with response to neoadjuvant immunotherapy in melanoma
    Article Snippet: The final nuclei suspensions were counted using Propidium Iodide staining solution (Invitrogen, REF# 3566) and the LUNA-FX7 Automated Cell Counter (Logos Biosystems). .. Single nuclei gene expression analysis was performed using the Chromium Next GEM single Cell Fixed RNA profiling assay (10x Genomics, PN-1000475), according to manufactures protocol CG000527. .. For each sample up to 200.000 nuclei were used as input for the barcoded probe hybridization using the Human Transcriptome Probe kit (10x Genomics, PN-1000420).

    Gene Expression:

    Article Title: Dysregulated lymphocyte localization in idiopathic multicentric Castleman disease
    Article Snippet: .. To determine the source of elevated chemokine expression in iMCD-TAFRO lymph nodes, we performed single-cell gene expression profiling of gently dissociated FFPE tissue using 10X Genomics’ FLEX pipeline. ..

    Article Title: Spatial cellular order underlies locally-confined mechanisms of immune resistance in oropharyngeal cancer.
    Article Snippet: .. Spatial gene expression profiling Xenium In Situ Technology from 10X Genomics was used to spatially detect gene expression using a custom probe set that included HPV16 genes (10X Genomics Custom Probe Set number 8XCY2D; Supplementary Dataset 1). ..

    Article Title: Longitudinal analysis reveals myeloid cell contributions to neuroPASC pathogenesis
    Article Snippet: Isolated cells were pelleted and fixed overnight in 10x Genomics Fixation Buffer (10x Genomics). .. Samples from 4–5 mice per experimental time point were pooled before submission to the University of Illinois Urbana-Champaign Genomics Core for processing with the 10x Genomics Chromium Fixed RNA Profiling (Flex) Gene Expression assay (10x Genomics). .. All libraries were prepared and sequenced together on the Illumina NovaSeq X Plus System, generating 28 × 90 nt reads.

    Article Title: Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging.
    Article Snippet: 29 Testicular aging is a key driver of declining male reproductive health, but a comprehensive 30 understanding of its underlying epigenetic drivers is lacking.. To address this, we construct a 31 multiomics aging atlas by integrating single-cell RNA sequencing, single-cell assay for 32 transposase-accessible chromatin sequencing (scATAC-seq), and spatial transcriptomics of 33 young and aged mouse testes.. Our analysis reveals that altered chromatin accessibility 34 accompanies transcriptional dysregulation and identifies spermatogonial stem cells (SSCs) as 35 the most epigenetically vulnerable population.

    Article Title: ROR1-PI3K/AKT signaling drives adaptive resistance to cell cycle blockade in TP53 mutated ovarian cancer
    Article Snippet: .. Single-cell gene expression profiling was conducted using the 10x Genomics Chromium Single Cell 3’RNAseq platform (10x Genomics, # CG000317 ). ..

    Article Title: CMAS dampens anti-tumor immunity and associates with response to neoadjuvant immunotherapy in melanoma
    Article Snippet: The final nuclei suspensions were counted using Propidium Iodide staining solution (Invitrogen, REF# 3566) and the LUNA-FX7 Automated Cell Counter (Logos Biosystems). .. Single nuclei gene expression analysis was performed using the Chromium Next GEM single Cell Fixed RNA profiling assay (10x Genomics, PN-1000475), according to manufactures protocol CG000527. .. For each sample up to 200.000 nuclei were used as input for the barcoded probe hybridization using the Human Transcriptome Probe kit (10x Genomics, PN-1000420).

    Article Title: Spatial atlas of human diabetic kidney uncovered podocyte-driven metabolic-inflammatory crosstalk via glycerolipid reprogramming and DUSP4/MMP3 axis
    Article Snippet: .. Visium HD spatial profiling was carried out on 6.5 mm2 slides (10x Genomics, PN-2000970) using the Human Spatial Gene Expression Reagent Kit (PN-1000675). ..

    Profiling:

    Article Title: Dysregulated lymphocyte localization in idiopathic multicentric Castleman disease
    Article Snippet: .. To determine the source of elevated chemokine expression in iMCD-TAFRO lymph nodes, we performed single-cell gene expression profiling of gently dissociated FFPE tissue using 10X Genomics’ FLEX pipeline. ..

    Article Title: CMAS dampens anti-tumor immunity and associates with response to neoadjuvant immunotherapy in melanoma
    Article Snippet: The final nuclei suspensions were counted using Propidium Iodide staining solution (Invitrogen, REF# 3566) and the LUNA-FX7 Automated Cell Counter (Logos Biosystems). .. Single nuclei gene expression analysis was performed using the Chromium Next GEM single Cell Fixed RNA profiling assay (10x Genomics, PN-1000475), according to manufactures protocol CG000527. .. For each sample up to 200.000 nuclei were used as input for the barcoded probe hybridization using the Human Transcriptome Probe kit (10x Genomics, PN-1000420).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Dysregulated lymphocyte localization in idiopathic multicentric Castleman disease
    Article Snippet: .. To determine the source of elevated chemokine expression in iMCD-TAFRO lymph nodes, we performed single-cell gene expression profiling of gently dissociated FFPE tissue using 10X Genomics’ FLEX pipeline. ..

    Article Title: CXCL10 drives female-specific tau pathology progression and defines sex-dependent vulnerability in tauopathy model mice
    Article Snippet: .. FFPE brain sections were analyzed using Xenium spatial transcriptome analysis (10x Genomics). .. FFPE brain sections were analyzed using Xenium spatial transcriptome analysis (10x Genomics).

    Tissue:

    Article Title: Dysregulated lymphocyte localization in idiopathic multicentric Castleman disease
    Article Snippet: .. To determine the source of elevated chemokine expression in iMCD-TAFRO lymph nodes, we performed single-cell gene expression profiling of gently dissociated FFPE tissue using 10X Genomics’ FLEX pipeline. ..

    In Situ:

    Article Title: Spatial cellular order underlies locally-confined mechanisms of immune resistance in oropharyngeal cancer.
    Article Snippet: .. Spatial gene expression profiling Xenium In Situ Technology from 10X Genomics was used to spatially detect gene expression using a custom probe set that included HPV16 genes (10X Genomics Custom Probe Set number 8XCY2D; Supplementary Dataset 1). ..

    Spatial Transcriptomics:

    Article Title: Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging.
    Article Snippet: 29 Testicular aging is a key driver of declining male reproductive health, but a comprehensive 30 understanding of its underlying epigenetic drivers is lacking.. To address this, we construct a 31 multiomics aging atlas by integrating single-cell RNA sequencing, single-cell assay for 32 transposase-accessible chromatin sequencing (scATAC-seq), and spatial transcriptomics of 33 young and aged mouse testes.. Our analysis reveals that altered chromatin accessibility 34 accompanies transcriptional dysregulation and identifies spermatogonial stem cells (SSCs) as 35 the most epigenetically vulnerable population.

    Sequencing:

    Article Title: Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging.
    Article Snippet: 29 Testicular aging is a key driver of declining male reproductive health, but a comprehensive 30 understanding of its underlying epigenetic drivers is lacking.. To address this, we construct a 31 multiomics aging atlas by integrating single-cell RNA sequencing, single-cell assay for 32 transposase-accessible chromatin sequencing (scATAC-seq), and spatial transcriptomics of 33 young and aged mouse testes.. Our analysis reveals that altered chromatin accessibility 34 accompanies transcriptional dysregulation and identifies spermatogonial stem cells (SSCs) as 35 the most epigenetically vulnerable population.

    Analysis:

    Article Title: CMAS dampens anti-tumor immunity and associates with response to neoadjuvant immunotherapy in melanoma
    Article Snippet: The final nuclei suspensions were counted using Propidium Iodide staining solution (Invitrogen, REF# 3566) and the LUNA-FX7 Automated Cell Counter (Logos Biosystems). .. Single nuclei gene expression analysis was performed using the Chromium Next GEM single Cell Fixed RNA profiling assay (10x Genomics, PN-1000475), according to manufactures protocol CG000527. .. For each sample up to 200.000 nuclei were used as input for the barcoded probe hybridization using the Human Transcriptome Probe kit (10x Genomics, PN-1000420).



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